MORPHOANATOMICAL AND PHYTOCHEMICAL STUDIES FOR THE QUALITY CONTROL OF EUPHORBIA LANCIFOLIA SCHLTDL. (EUPHORBIACEAE)

Authors

  • M. E. Paredes Escuela de Química Biológica, Facultad de Ciencias Químicas y Farmacia, Universidad de San Carlos de Guatemala, Guatemala.
  • R. E. Morales Coromac Escuela de Química Biológica, Facultad de Ciencias Químicas y Farmacia, Universidad de San Carlos de Guatemala, Guatemala.
  • W. C. Lima Ortiz Escuela de Química Biológica, Facultad de Ciencias Químicas y Farmacia, Universidad de San Carlos de Guatemala, Guatemala
  • S. Hu, MPharm Department of Pharmaceutical and Biological Chemistry, UCL School of Pharmacy, United Kingdom.
  • A. L. Valle Jurado Facultad de Biología, Química y Farmacia (FABIQ), Universidad Galileo, Guatemala
  • O. Farsi Laboratorios QUINFICA, Guatemala
  • D. Farchi Laboratorios QUINFICA, Guatemala
  • J. M Prieto School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University

DOI:

https://doi.org/10.4314/jfas.v13i3.9

Keywords:

HPLC, TLC, Microscopy, Macroscopy, Euphorbia lancifolia, Guatemala

Abstract

Introduction: Euphorbia lancifolia Schltdl. (Euphorbiaceae) is a medicinal specie of central America origin, known as Ixbut in Guatemala, widely recognized for its important natural galactogogue activity. Methods: as identity test we here run macro and micro morphoanatomical studies of the characters of the vegetative organs. We also developed standard chemical tests for quality by both TLC and HPLC for infusions and tinctures of varying alcoholic strength. Their radical scavenging activities in DPPH and NO were also measured. Results: macro and micro morphoanatomical characters of the vegetative organs present a set of characteristics to facilitate the identification of dry powdered samples of this species. We developed optimal conditions for the TLC and HPLC phytochemical fingerprints of the 4 most common pharmacopoeial liquid herbal preparations from this herbal drug, namely infusion, 70%, 45% and 20% hydroalcoholic tinctures. Conclusions: Our work provides the Latin-American industry with a set of analyses to establish the identity and chemistry of E. lancifolia samples for quality control purposes

Downloads

Download data is not yet available.

References

Rosengarten, F. Jr. A neglected Mayan galactagogue - ixbut (Euphorbia lancifolia). J. Ethnopharmacol, 1982, 5, 91 – 112.

Cáceres, A, Vademécum nacional de plantas medicinales. Ed. Universidad de San Carlos de Guatemala, Guatemala. Ministerio de Salud Pública y Asistencia Social. Editorial Universitaria, Universidad de San Carlos de Guatemala, 2009.

Standley, P.C., Steyermark, JA, and Steyermark, J.A. Flora of Guatemala. Ed. Standley PC and Steyermark J.A. Chicago Natural History Museum, Chicago, 1949.

Cates, R. G., Thompson, A. , Brabazon, H., McDonald, S., Lawrence, M., Williams, S., Peniallilo, P., Fuentes Soria, J. A., Espinoza, L V., Martinez J. V., Arbizú, D. A., Villagran, E. and F Ancheta. Activities of Guatemalan medicinal plants against cancer cell lines and selected microbes: Evidence for their conservation. J Med Plant Res, 2014, 8(33), 1040-1050.

The Plant List. 2018. URL: http://www.theplantlist.org

Caceres, A., Michel, J.L., Doyle, B.J., Locklear, T.D. and Mahady, G,B. Estrogenic and Progestagenic Effects of Medicinal Plants Used for Women’s Reproductive Health in Guatemala. Planta Med 2014, 80(10), CL2.

Jewell, L.E, Synthesis of glycolipids inspired by Fucus distichus and natural product isolation and characterization of Euphorbia lancifolia. Thesis, University of Otawa, 2009.

MINECO. Productos farmaceuticos. Productos naturales medicinales para uso humano. Verificacion de la Calidad. Regl. Tec. Centroam. 11035609 (270). Guatemala, Guatemala, 2011.

Prieto, J.M. Garantia de calidad para productos naturales: oportunidades y desafios para paises en desarrollo. Workshop. Organised by: QUINFICA, AFITOGUA y Universidad Galileo. Universidad Galileo, Guatemala City, 30-31 March 2018.

European Directorate for the Quality of Medicines & HealthCare. European Pharmacopoeia 7.0. 2010, 519.

Sólis, P., De Solis, N., Gattuso, S., and Cáceres, A. Manual de caracterización y análisis de drogas vegetales y productos fitoterapéuticos. Proyecto de Desarrollo y Tecnología de Cultivo de Plantas Medicinales y Producción de Fitoterápicos. 2003Proyecto d. OEA/AICD/AE 089/03.

Gattuso, M., and Gattuso, S. Manual de Procedimientos para el análisis de drogas en polvo. Universidad Nacional De Rosario, Rosario, Argentina, 1999.

Giner, R.M., Recio, M.C., Cuellar, M.J., Manez, S., Peris, J.B., Stubing, G., and Rios, J.L.. A taxonomical study of the subtribe leontodontinae based on the distribution of phenolic compounds. Biochem Syst Ecol, 1993, 21(5), 613–616.

Burits, M., and Bucar, F. Antioxidant activity of Nigella sativa essential oil. Phytother Res, 2000, 14(5), 323–328.

Sreejayan, R.M.N.A. Nitric oxide scavenging by curcuminoids. J Pharm Pharmacol, 1997, 49(1):105–107.

Prior RL, Wu X, and Schaich K. Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. J Agric Food Chem, 2005, 53(10), 4290–4302.

Moawed, M.M., Saaid, S., Abdelsamie, Z., Tantawy, M. Phenetic analysis of certain taxa of Euphorbiaceae grown in Egypt. Egypt J Bot, 2015, 55(2), 247-267.

Marchessi, J.E., Subils, R., Scaramuzzino, R.L., Crosta, H.N., Eseiza, M.F., Saint, A.H.M., and Juan, V.F. Presencia de Euphorbia davidii Subils (Euphorbiaceae) en la Provincia de Buenos Aires: morfología y anatomía de la especie. Kurtziana, 2011, 36 (1), 45-53.

Inamdar, J.A. and Gangadhara, M. Structure and ontogeny of stomata in some Euphorbiaceae. Phyton, 1978, 19(1-2), 37-60.

Devi, N., Padma, Y., Narasimhudu, C., and Raju, R. Diversity of stomata and trichomes in Euphorbia L. - I. Bangl J Plant Taxon, 2013, 20(1), 27-38.

Downloads

Published

2021-07-28

How to Cite

PAREDES, M. E.; MORALES COROMAC, R. E.; LIMA ORTIZ, W. C.; HU, S.; VALLE JURADO, A. L.; FARSI, O.; FARCHI, D.; PRIETO, J. M. MORPHOANATOMICAL AND PHYTOCHEMICAL STUDIES FOR THE QUALITY CONTROL OF EUPHORBIA LANCIFOLIA SCHLTDL. (EUPHORBIACEAE). Journal of Fundamental and Applied Sciences, [S. l.], v. 13, n. 3, p. 1285–1301, 2021. DOI: 10.4314/jfas.v13i3.9. Disponível em: https://www.jfas.info/index.php/JFAS/article/view/874. Acesso em: 12 nov. 2024.

Issue

Section

Articles