PREPARATION, CHARACTERIZATION AND IN VITRO ANTIMICROBIAL ACTIVITY EVALUATION OF THYME ESSENTIAL OIL FORMULATION TO TREAT SKIN INFECTIONS
DOI:
https://doi.org/10.4314/jfas.v12i1S.1Keywords:
Thyme; essential oil; antimicrobial activity; skin irritation; cutaneous infections.Abstract
The aim of the present study was to develop and evaluate a topical formulation containing Thymus fontanesii Boiss. & Reut essential oil that could serve as an antimicrobial agent for the treatment of various skin diseases caused by bacteria, yeast and dermatophytes. The selected formulation exhibited a significant antimicrobial activity against all tested strains, better consistency, good homogeneity, no skin irritation and good stability for six months at 25 ± 2°C. Our results demonstrate that the formulation could be used safely as topical preparation to treat skin diseases caused by the tested microorganisms into the future.
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References
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[26] Adams RP. Identification of Essential oil Components by gas Chromatography/ Mass Spectrometry. In: IL UCS, editor.2007.
[27] El Ouariachi el M, Tomi P, Bouyanzer A, Hammouti B, Desjobert JM, Costa J, Paolini J. Chemical composition and antioxidant activity of essential oils and solvent extracts of Ptychotis verticillata from Morocco. Food Chem Toxicol, 2011,49(2),533-536.
[28] Kohiyama CY, Yamamoto Ribeiro MM, Mossini SA, Bando E, Bomfim Nda S, Nerilo SB, Rocha GH, Grespan R, Mikcha JM, Machinski M, Jr. Antifungal properties and inhibitory effects upon aflatoxin production of Thymus vulgaris L. by Aspergillus flavus Link. Food Chem, 2015,173,1006-1010.
[29] Mouraviev IA. Technologie des formes médicamenteuses. Medecine edMoscou,1988,pp. 200- 224.
[30] Builders PF, Kabele-Toge B, Builders M, Chindo BA, Anwunobi PA, Isimi YC. Wound Healing Potential of Formulated Extract from Hibiscus Sabdariffa Calyx. Indian J Pharm Sci, 2013,75(1),45-52.
[31] Gupta M, Vyas SP. Development, characterization and in vivo assessment of effective lipidic nanoparticles for dermal delivery of fluconazole against cutaneous candidiasis. Chem Phys Lipids, 2012,165(4),454-461.
[32] Pople PV, Singh KK. Development and Evaluation of Topical Formulation Containing Solid Lipid Nanoparticles of Vitamin A. AAPS PharmSciTech, 2006,7(4).
[2] Viswanad V, Aleykutty NA, Jayakar B, Zacharia SM, Thomas L. Development and evaluation of antimicrobial herbal formulations containing the methanolic extract of Samadera indica for skin diseases. J Adv Pharm Technol Res, 2012,3(2),106-111.
[3] Barros L, Heleno SA, Carvalho AM, Ferreira ICFR. Lamiaceae often used in Portuguese folk medicine as a source of powerful antioxidants: Vitamins and phenolics. LWT - Food Science and Technology, 2010,43(3),544-550.
[4] Djenane D, Aider M, Yanguela J, Idir L, Gomez D, Roncales P. Antioxidant and antibacterial effects of Lavandula and Mentha essential oils in minced beef inoculated with E. coli O157:H7 and S. aureus during storage at abuse refrigeration temperature. Meat Sci, 2012,92(4),667-674.
[5] Hosni K, Hassen I, Chaâbane H, Jemli M, Dallali S, Sebei H, Casabianca H. Enzyme-assisted extraction of essential oils from thyme (Thymus capitatus L.) and rosemary (Rosmarinus officinalis L.): Impact on yield, chemical composition and antimicrobial activity. Industrial Crops and Products, 2013,47,291-299.
[6] Ferhat M, Ghorab H, Laggoune S, Ghannadi A, Sajjadi S, Touzani R, Kabouche AaK, Z. Composition and antioxidant activity of the essential oil of Thymus dreatensis from Algeria. Chemistry of Natural Compounds, 2014,50(4),747-749.
[7] Ghannadi A, Sajjadi SE, Kabouche A, Kabouche Z. Thymus fontanesii Boiss. & Reut.-A potential source of thymol-rich essential oil in North Africa. Z Naturforsch C, 2004,59,187-189.
[8] Kabouche Z, Boutaghane N, Laggoune S, Kabouche A, Aitkaki Z, Benlabed K. Comparative antibacterial activity of five Lamiaceae essential oils from Algeria. International Journal of Aromatherapy, 2005,15(3),129-133.
[9] Haddouchi F, Lazouni HA, Meziane A, Benmansour A. Study physicochemical and microbiological of the essential oil of Thymus fontanesii Boiss & Reut. Afrique SCIENCE, 2009,05(2),246 - 259.
[10] Mohammedi Z, Bachik S, Belkaroube N. Antifungal and antiaflatoxigenic potential of essential oils from an endemic Thymus fontanesii Boiss and Reut. Les technologies de laboratoire, 2010,5(19),10-15.
[11] Khadir A, Bendahou M, Benbelaid F, Abdoune MA, Abdelouahid DE. Pouvoir antimicrobien de Thymus lanceolatus Desf., récolté en Algérie. Phytothérapie, 2013,11(6),353-358.
[12] Dob T, Dahmane D, Benabdelkader T, Chelghoum C. Composition and antimicrobial activity of the essential oil of Thymus fontanesii. Pharmaceutical Biology, 2008,44(8),607-612.
[13] Matias EFF, Alves EF, Silva MKN, Carvalho VRA, Figueredo FG, Ferreira JVA, Coutinho HDM, Silva JMFL, Ribeiro-Filho J, Costa JGM. Seasonal variation, chemical composition and biological activity of the essential oil of Cordia verbenacea DC (Boraginaceae) and the sabinene. Industrial Crops and Products, 2016,87,45-53.
[14] Llorens L, Llorens-Molina JA, Agnello S, Boira H. Geographical and environment-related variations of essential oils in isolated populations of Thymus richardii Pers. in the Mediterranean basin. Biochemical Systematics and Ecology, 2014,56,246-254.
[15] Karami MA, Moghimipour E, Saafi SF. Preparation and evaluation of Cordia myxa fruit topical cream. World Journal of Pharmaceutical Research, 2015,4 (7),244-253.
[16] Raghavamma STV, Anjali MV, Amrutha NN, Hemalatha T, Rao Nadendla R. Assessment of Efficacy and Safety of Newly Formulated Natural Sun Protective Cream. IOSR Journal of Pharmacy and Biological Sciences, 2016,11(05),119-124.
[17] Aslani A, Zolfaghari B, Davoodvandi F. Design, Formulation and Evaluation of an Oral Gel from Punica Granatum Flower Extract for the Treatment of Recurrent Aphthous Stomatitis. Adv Pharm Bull, 2016,6(3),391-398.
[18] Ortega YH, Mosquera DMG, Galapththi S, Santos BT, Gonzalez GS, Foubert K, Pieters L, Calderon PB, Apers S. Safety assessment of a traditionally used extract from leaves of Boldoa purpurascens. J Ethnopharmacol, 2016,192,302-308.
[19] Djabou N, Lorenzi V, Guinoiseau E, Andreani S, Giuliani M-C, Desjobert J-M, Bolla J-M, Costa J, Berti L, Luciani A, Muselli A. Phytochemical composition of Corsican Teucrium essential oils and antibacterial activity against foodborne or toxi-infectious pathogens. Food Control, 2013,30(1),354-363.
[20] Ballester-Costa C, Sendra E, Fernández-López J, Pérez-Álvarez JA, Viuda-Martos M. Chemical composition and in vitro antibacterial properties of essential oils of four Thymus species from organic growth. Industrial Crops and Products, 2013,50,304-311.
[21] Fadli M, Saad A, Sayadi S, Chevalier J, Mezrioui NE, Pages JM, Hassani L. Antibacterial activity of Thymus maroccanus and Thymus broussonetii essential oils against nosocomial infection - bacteria and their synergistic potential with antibiotics. Phytomedicine, 2012,19(5),464-471.
[22] Pinto E, Pina-Vaz C, Salgueiro L, Goncalves MJ, Costa-de-Oliveira S, Cavaleiro C, Palmeira A, Rodrigues A, Martinez-de-Oliveira J. Antifungal activity of the essential oil of Thymus pulegioides on Candida, Aspergillus and dermatophyte species. J Med Microbiol, 2006,55(Pt 10),1367-1373.
[23] Adrar N, Oukil N, Bedjou F. Antioxidant and antibacterial activities of Thymus numidicus and Salvia officinalis essential oils alone or in combination. Industrial Crops and Products, 2016,88,112-119.
[24] Hajlaoui H, Mighri H, Aouni M, Gharsallah N, Kadri A. Chemical composition and in vitro evaluation of antioxidant, antimicrobial, cytotoxicity and anti-acetylcholinesterase properties of Tunisian Origanum majorana L. essential oil. Microb Pathog, 2016,95,86-94.
[25] de Carvalho RJ, de Souza GT, Honorio VG, de Sousa JP, da Conceicao ML, Maganani M, de Souza EL. Comparative inhibitory effects of Thymus vulgaris L. essential oil against Staphylococcus aureus, Listeria monocytogenes and mesophilic starter co-culture in cheese-mimicking models. Food Microbiol, 2015,52,59-65.
[26] Adams RP. Identification of Essential oil Components by gas Chromatography/ Mass Spectrometry. In: IL UCS, editor.2007.
[27] El Ouariachi el M, Tomi P, Bouyanzer A, Hammouti B, Desjobert JM, Costa J, Paolini J. Chemical composition and antioxidant activity of essential oils and solvent extracts of Ptychotis verticillata from Morocco. Food Chem Toxicol, 2011,49(2),533-536.
[28] Kohiyama CY, Yamamoto Ribeiro MM, Mossini SA, Bando E, Bomfim Nda S, Nerilo SB, Rocha GH, Grespan R, Mikcha JM, Machinski M, Jr. Antifungal properties and inhibitory effects upon aflatoxin production of Thymus vulgaris L. by Aspergillus flavus Link. Food Chem, 2015,173,1006-1010.
[29] Mouraviev IA. Technologie des formes médicamenteuses. Medecine edMoscou,1988,pp. 200- 224.
[30] Builders PF, Kabele-Toge B, Builders M, Chindo BA, Anwunobi PA, Isimi YC. Wound Healing Potential of Formulated Extract from Hibiscus Sabdariffa Calyx. Indian J Pharm Sci, 2013,75(1),45-52.
[31] Gupta M, Vyas SP. Development, characterization and in vivo assessment of effective lipidic nanoparticles for dermal delivery of fluconazole against cutaneous candidiasis. Chem Phys Lipids, 2012,165(4),454-461.
[32] Pople PV, Singh KK. Development and Evaluation of Topical Formulation Containing Solid Lipid Nanoparticles of Vitamin A. AAPS PharmSciTech, 2006,7(4).
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Published
2019-11-07
How to Cite
MEBARKI, N.; NABIEV, M.; ZIANE, H.; CHADER, H.; FAZOUANE, F. PREPARATION, CHARACTERIZATION AND IN VITRO ANTIMICROBIAL ACTIVITY EVALUATION OF THYME ESSENTIAL OIL FORMULATION TO TREAT SKIN INFECTIONS. Journal of Fundamental and Applied Sciences, [S. l.], v. 12, n. 1S, p. 1–16, 2019. DOI: 10.4314/jfas.v12i1S.1. Disponível em: https://www.jfas.info/index.php/JFAS/article/view/590. Acesso em: 24 mar. 2023.
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