• M. Beghalia Institute of Sciences and Technology, University Centre of Tissemsilet Algeria
  • S. Ghalem LASNABIO. Department of Chemistry, Faculty of Sciences, University of Tlemcen, Algeria
  • H. Allali LASNABIO. Department of Chemistry, Faculty of Sciences, University of Tlemcen, Algeria



Key words Crystallization , Inhibitors , Globularia , lithiasis , Artificial urine


Plant extracts are known to modulate calcium oxalate (CaOx) crystallization. One of these is Globularia, the root and flower. Medicinals plants has been demonstrated to be an efficient inhibitor of CaOx crystal growth; however, its inhibitory activity against other events in CaOx crystallization has not been fully investigated. To assess the potential of Globularia as an effective inhibitor, its effects on CaOx crystal nucleation and aggregation were evaluated. Nucleation and aggregation of CaOx crystals were studied by artificial urine is prepared by mixing and stirring two equal volumes of 50 ml of solutions A and B at constant temperature (37°C). In the nucleation assay, crystallization was induced by mixing calcium chloride and sodium oxalate, The addition of  extracts Globularia flower and root acts on the phase of growth  crystallisation ; the rate of inhibition capacity in concentration 100 % is 96.09% , and 96.77% for concentration 75% respectively, On note that  these inhibitors developed an inhibition important on the low concentration. Globularia is an efficient inhibitor of crystal nucleation and aggregation. Its presence in the kidneys and urine may protect subjects against CaOx crystallization and kidney stone formation.


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How to Cite

BEGHALIA, M.; GHALEM, S.; ALLALI, H. ROLE GLOBULARIA IN THE INHIBITION OF CALCIUM OXALATE CRYSTALLIZATION IN VITRO STUDY. Journal of Fundamental and Applied Sciences, [S. l.], v. 12, n. 2, p. 845–851, 2020. DOI: 10.4314/jfas.v12i2.21. Disponível em: Acesso em: 25 feb. 2024.