VALORIZATION OF CITRUS SINENSIS WASTES FOR THE PRODUCTION OF ORANGE ESSENTIAL OIL: IDENTIFICATION OF CHEMICAL COMPOSITION AND ANTIBACTERIAL ACTIVITY

Lamria  Seid; Delloula  Lakhdari; Ouafia Belgherbi; Ines  Laourari; Nasrin  Golzadeh; Dalila  Chouder

doi:10.4314/jfas.1252

Authors

Lamria Seid Laboratoire LEES, Faculté de Technologie, Université Sétif-1, Alegria
Delloula Lakhdari Research Center in Industrial Technologies CRTI, P.O. Box 64، Cheraga 16014, Algeria
Ouafia Belgherbi Research Center in Industrial Technologies CRTI, P.O. Box 64, Cheraga 16014, Algiers, Algeria
Ines Laourari Laboratoire Biotechnologies, Ecole Nationale Supérieure de Biotechnologie, Ville Universitaire Ali Mendjeli, BP E66 25100, Constantine, Algeria
Nasrin Golzadeh STEM Knowledge Translations Institute, Montreal, Quebec, Canada
Dalila Chouder Laboratoire LEES, Faculté de Technologie, Université Sétif-1, Alegria

DOI:

https://doi.org/10.4314/jfas.1252

Keywords:

Citrus sinensis, Orange essential oil, Antibacterial activity, Staphylococcus aureus, Escherichia coli, Pseudomonas aerogenes

Abstract

The objective of this study was to extract, characterize, and examine the antibacterial properties of orange essential oil obtained from citrus sinensis peels, using hydro-distillation method. Various components of the orange oil were characterized by FTIR, UV-Vis and GC- MS spectroscopic techniques. The extracted essential oil was conformed to AFNOR standards, and had a maximum yield of 3.4% (w/w). The obtained results showed that limonene was the major bioactive components of the orange essential oil (97.14%). The essential orange oil and its dilutions in DMSO showed an excellent antibacterial activity against Staphylococcus aureus (gram-positive bacteria) with a diameter of inhibition zone superior to 40 mm. However, it was ineffective antibacterial activity against Escherichia coli and Pseudomonas aerogenes (gram negative bacteria).

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