EFFECT OF DENATURING AGENTS ON A LECTIN ISOLATED FROM MARINE SPONGE AXINELLA DONNANI AND ITS POTENTIAL CYTOTOXIC ACTIVITY

Authors

  • Ratheesh Sadanandan Department of Biochemistry, University of Kerala, Trivandrum-695581, Kerala, India
  • Arun A. Rauf Department of Biochemistry, University of Kerala, Trivandrum-695581, Kerala, India

DOI:

https://doi.org/10.4314/jfas.1195

Keywords:

Axinella donnani, Lectin, MCF-7, A431, HeLa, MTT assay

Abstract

Marine sponges are oldest metazoans which were found to have structural and biologically diverse lectins. A lectin isolated from marine sponge Axinella donnani (ADL) were evaluated for its stability against denaturing agents such as Urea, Thiourea and Guanidine hydrochloride. Urea and Thiourea decreases the stability of ADL in higher concentration (5 mM) while Guanidine hydrochloride does not show any activity. Finally, cytotoxicity of ADL at different concentrations (0.25-500 mg/mL) was tested against MCF-7, A431 and HeLa cell lines by MTT assay. ADL exhibited potential cytotoxicity against these cell lines after 24 hours in a dose dependent manner. Thus, ADL could be a potent cytotoxic agent in clinical applications.

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Published

2022-04-30

How to Cite

SADANANDAN, R.; A. RAUF, A. EFFECT OF DENATURING AGENTS ON A LECTIN ISOLATED FROM MARINE SPONGE AXINELLA DONNANI AND ITS POTENTIAL CYTOTOXIC ACTIVITY. Journal of Fundamental and Applied Sciences, [S. l.], v. 14, n. 2, p. 312–319, 2022. DOI: 10.4314/jfas.1195. Disponível em: https://www.jfas.info/index.php/JFAS/article/view/1195. Acesso em: 30 jan. 2023.

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